Effects of glyphosate on gene expression and its implications for human health

Authors

  • Ana M. Lara-Rodríguez Universidad Antonio Nariño
  • Valentina Páez-Almanza Universidad Antonio Nariño
  • Oriana V. Manrique-Torres Universidad Antonio Nariño
  • César J. Guevara-Medina Universidad Antonio Nariño
  • Andrés F. Alarcón Vargas Universidad Antonio Nariño
  • Nelson E. Arenas Universidad Antonio Nariño https://orcid.org/0000-0002-7665-8955
  • Laura I. Cuervo-Soto Universidad Antonio Nariño

DOI:

https://doi.org/10.36436/24223484.328

Keywords:

Herbicidas, agrotóxicos, salud ambiental, glifosato, cultivos ilícitos.

Abstract

Glyphosate is a herbicide that has caused considerable environmental and political controversy due to its use in the eradication of illicit crops in Colombia. Our goal was to provide technical elements based on scientific studies to contribute to the knowledge demonstrating the genotoxic effect of glyphosate on human health and some mammalian species. The studies found evidence a series of effects at the cellular level in different biological systems and changes at the epigenetic level. Additionally, glyphosate affects survival due to changes in cell cycle regulation and metabolism, disruption of DNA repair, accumulation of mutations that could lead to cell death, and tumorigenesis.

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Author Biography

Ana M. Lara-Rodríguez, Universidad Antonio Nariño

Biochemistry Program, Faculty of Sciences, Antonio Nariño University, Bogotá (Colombia).

References

Andreotti G, Koutros S, Hofmann JN, Sandler DP, Lubin JH, Lynch CF, … Beane Freeman LE. Glyphosate Use and Cancer Incidence in the Agricultural Health Study. JNCI: Journal of the National Cancer Institute, 2017;110(5):509–516.

Woźniak E, Reszka E, Jabłońska E, Balcerczyk A, Broncel M, Bukowska B. Glyphosate affects methylation in the promoter regions of selected tumor suppressors as well as expression of major cell cycle and apoptosis drivers in PBMCs (in vitro study). Toxicology in Vitro. 2020;63:104736.

Agostini L, Dettogni RS, dos Reis RS, Stur E, dos Santos EV, Ventorim DP, ...Louro ID. Effects of glyphosate exposure on human health: Insights from epidemiological and in vitro studies. Sci. Total Environ. 2020;705:135808.

Kahl VF, Dhillon V, Fenech M, de Souza MR, da Silva FN, Marroni NA, … da Silva J. Occupational Exposure to Pesticides in Tobacco Fields: The Integrated Evaluation of Nutritional Intake and Susceptibility on Genomic and Epigenetic Instability. Oxid. Med. Cell. Longev, 2018;1–13.

Jayasumana C, Gunatilake S, Senanayake P. Glyphosate, hard water and nephrotoxic metals: Are they the culprits behind the epidemic of chronic kidney disease of unknown etiology in Sri Lanka? Int. J. Environ. Res. Public Health, 2014;11(2):2125–2147.

De Arauj JS, Delgado IF, Paumgartten FJ. Glyphosate and adverse pregnancy outcomes, a systematic review of observational studies. BMC Public Health, 2016;16:1.

Alvarez-Moya C, Reynoso Silva M, Valdez-Ramírez C, Gómez-Gallardo D, León Sánchez R, Canales-Aguirre A et al. Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms. Genet. Mol. Biol. 2014;37(1):105-110.

Kwiatkowska M, Reszka E, Woźniak K, Jabłońska E, Michałowicz J, Bukowska B. DNA damage and methylation induced by glyphosate in human peripheral blood mononuclear cells (in vitro study). Food Chem. Toxicol, 2017;105:93-98.

Woźniak E, Sicińska P, Michałowicz J, Woźniak K, Reszka E, Huras B, ...Bukowska B. The mechanism of DNA damage induced by Roundup 360 PLUS, glyphosate and AMPA in human peripheral blood mononuclear cells-genotoxic risk assessement. Food Chem. Toxicol, 2018;120:510-522.

Elie-Caille C, Heu C, Guyon C, Nicod L. Morphological damages of a glyphosate-treated human keratinocyte cell line revealed by a micro-to nanoscale microscopic investigation. Cell Biol Toxicol, 2010;26(4):331-339.

Mañas F, Peralta L, Raviolo J, Ovando HG, Weyers A, Ugnia L, ... Gorla, N. Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests. Environ. Toxicol. Pharmacol, 2009;28(1):37-41.

Stur E, Aristizabal-Pachon AF, Peronni KC, Agostini LP, Waigel S, Chariker J, et al. Glyphosate-based herbicides at low doses affect canonical pathways in estrogen positive and negative breast cancer cell lines. PLoS ONE 2019;14(7): e0219610.

Kašuba V, Milić M, Rozgaj R, Kopjar N, Mladinić M, Žunec S, ... Lovaković BT. Effects of low doses of glyphosate on DNA damage, cell proliferation and oxidative stress in the HepG2 cell line. Environ Sci Pollut Res Int, 2017;24(23):19267-19281.

Hao Y, Chen H, Xu W, Gao J, Yang Y, Zhang Y, Tao L. Roundup® confers cytotoxicity through DNA damage and Mitochondria-Associated apoptosis induction. Environmental pollution, 2019;252:917-923.

Nagy K, Tessema RA, Budnik LT, Ádám B. Comparative cyto-and genotoxicity assessment of glyphosate and glyphosate-based herbicides in human peripheral white blood cells. Environ. Res., 2019;179:108851.

Jeon, Lee, SH, Roh J, Kim JE, Bunch H. Glyphosate influences cell proliferation in vitro. All Life, 2020;13(1):54-65.

Pourahmad J, Salimi A. Isolated Human Peripheral Blood Mononuclear Cell (PBMC), a Cost-Effective Tool for Predicting Immunosuppressive Effects of Drugs and Xenobiotics. IJPR, 2015;14(4):979.

Portier CJ. A comprehensive analysis of the animal carcinogenicity data for glyphosate from chronic exposure rodent carcinogenicity studies. Environmental Health, 2020;19(1):18.

Townsend M, Peck C, Meng W, Heaton M, Robison R, O'Neill K. Evaluation of various glyphosate concentrations on DNA damage in human Raji cells and its impact on cytotoxicity. Regul. Toxicol. Pharmacol., 2017;85:79-85.

Thongprakaisang S, Thiantanawat A, Rangkadilok N, Suriyo T, Satayavivad J. Glyphosate induces human breast cancer cells growth via estrogen receptors. Food Chem Toxicol., 2013;59:129-136.

Hao Y, Xu W, Gao J, Zhang Y, Yang Y, Tao L. Roundup-induced AMPK/mTOR-mediated autophagy in human A549 cells. J. Agric. Food Chem., 2019;67(41):11364-11372.

Rueda-Ruzafa L, Cruz F, Roman P, Cardona D. Gut microbiota and neurological effects of glyphosate. Neurotoxicology, 2019;75:1-8.

Instituto Nacional de Salud, Observatorio Nacional de Salud, Consecuencias del Conflicto Armado en Salud en Colombia; Noveno Informe Técnico, 340 p. Bogotá, D.C., 2017. Disponible en: https://www.ins.gov.co/Direcciones/ONS/Informes/9%20Consecuencias%20del%20Conflicto%20Armado%20en%20la%20Salud%20en%20Colombia.pdf

Espinosa JE. Comentario, El glifosato no es la solución para Colombia. The New York Times, 15 de Abril de 2019. Disponible en: https://www.nytimes.com/es/2019/04/15/espanol/opinion/glifosato-colombia-duque.html Accedido: 04/12/2020.

Camacho A, Mejía D. Consecuencias de la aspersión aérea en la salud: evidencia desde el caso colombiano. In: Andes U de los, editor. Costos económicos y sociales del conflicto en Colombia ¿Cómo construir un posconflicto sostenible? 2014. p. 270.

Camacho GS, Herrera AV, Rincón DM, Ospina JE. Efectos del glifosato sobre la salud humana. El Centauro 2016;8(11):71-86.

Varona M, Henao GL, Díaz S, Lancheros A, Murcia Á, Rodríguez N, Álvarez VH. Evaluación de los efectos del glifosato y otros plaguicidas en la salud humana en zonas objeto del programa de erradicación de cultivos ilícitos. Biomédica, 2009;29(3):456-475.

Moreno T. Caso probable de muerte por glifosato relacionado con el programa de erradicación de cultivos ilícitos en la vereda La Guayacana, Tumaco, Nariño, abril de 2008. Informe Quincenal Epidemiológico, Instituto Nacional de Salud. 2008;13(12):177-183.

Avila-Vazquez M, Maturano E, Etchegoyen MA, Difilippo FS, Maclean B. Association between cancer and environmental exposure to glyphosate. IJCM 2017;8(2):73-85.

Chaves VS, Islam MT, de Moraes MO, de Alencar MV, Gomes CV, de Carvalho RM, ...Rolim ML. Occupational and life-style factors-acquired mutagenicity in agric-workers of northeastern Brazil. Environ Sci Pollut Res Int., 2017;24(18):15454-15461.

Avila-Vazquez M, Difilippo FS, Mac-Lean B, Maturano E, Etchegoyen A. Environmental exposure to glyphosate and reproductive health impacts in agricultural population of Argentina. J Environ Prot., 2018;9(03):241.

Zhao J, Pacenka S, Wu J, Richards BK, Steenhuis T, Simpson K, Hay AG. Detection of glyphosate residues in companion animal feeds. Environ. Pollut., 2018;243:1113-1118.

Martin-Culma NY, Arenas NE. Daño colateral en abejas por la exposición a pesticidas de uso agrícola. Entramado., 2018;14(1):232-240.

Leoci R, Ruberti M. Glyphosate in agriculture: Environmental persistence and effects on animals. A Review. J. Agr. Env. Int. Dev. 2020;114(1):99-122.

Gill, PK, Sethi N, Mohan A, Datta S, Girdhar M. Glyphosate toxicity for animals. Environ Chem Lett., 2018;16(2):401-426.

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Published

2020-12-30

How to Cite

Lara-Rodríguez, A. M., Páez-Almanza, V., Manrique-Torres, O. V., Guevara-Medina, C. J., Alarcón Vargas, A. F., Arenas, N. E., & Cuervo-Soto, L. I. (2020). Effects of glyphosate on gene expression and its implications for human health. Ciencias Agropecuarias, 6(2), 71–82. https://doi.org/10.36436/24223484.328

Issue

Vol. 6 No. 2 (2020)

Section

Articles

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