Use of selective media as a preliminary test for the search for Salmonella spp. and Escherichia coli in tap water at the University of Cundinamarca's main campus and La Esperanza farm.
DOI:
https://doi.org/10.36436/24223484.218Keywords:
Salmonella, recuento en placa, agar verde brillante.Abstract
Selective media (Salmonella-Shiguella Agar, Eosin Methylene Blue, and Brilliant Green Agar) were used to evaluate the presence of pathogenic bacteria in water samples previously collected from a bathroom faucet at the University of Cundinamarca Main Campus and La Esperanza Farm. Water samples were taken in the morning (9:00 am) and in the afternoon (2:00 pm). The number of bacterial colonies present in the samples was determined by plate count, and the samples were plated on the aforementioned selective media. No bacterial colonies were found in the water samples taken at the University Main Campus, but presumptive growth of Salmonella bacteria and Escherichia coli was found on the brilliant green agar in the samples taken from the La Esperanza Farm faucet in the afternoon.Downloads
References
Pimentel D, Berger B, Filiberto D, Newton M, Wolfe B, Karabinakis E, et al. Water Resources : Agricultural and Environmental Issues. 2013;54(10):909–18.
ONU. La situación La crisis mundial del agua [Internet]. UNESCO/Mundi-Prensa Libros, editor. Agua para todos, agua para la vida. Informe de las Naciones Unidas sobre el desarrollo de los recursos hídricos en el mundo. 2003. Available from: http://www.un.org/esa/sustdev/sdissues/water/WWDRspanish-129556s.pdf
Proctor CR, Hammes F. Drinking water microbiology — from measurement to management. Curr Opin Biotechnol [Internet]. 2015;33(Figure 1):87–94. Available from: http://dx.doi.org/10.1016/j.copbio.2014.12.014
Campos-Pinilla C, Cárdenas-Guzmán M, Guerrero Cañizares A. Performance of Faecal Contamination Indicators in Different Type of Waters From the Sabana of Bogotá ( Colombia ). Univ Sci. 2008;13:103–8.
Rojas-triviño A. Conceptos y práctica de microbiología general. Universidad Nacional de Colombia. 2011. 1-161 p.
Atlas R. Handbook of Microbiological Media, Fourth Edition [Internet]. 2010. 978-1-4398-0408-7 (EbookPDF) p. Available from: http://www.crcnetbase.com/doi/book/10.1201/EBK1439804063
Beckton Dickinson. BBLTM Brilliant Green Agar [Internet]. 2012. Available from: https://www.bd.com/ds/technicalCenter/inserts/8806221(02)(201202).pdf
Koneman E (et al). Koneman Diagnóstico microbiológico: texto y atlas en color. 6th ed.
Ratnayaka DD, Brandt MJ, Johnson KM. {CHAPTER} 6 - Chemistry, Microbiology and Biology of Water. In: Water Supply (Sixth Edition) [Internet]. 2009. p.195–266. Available from: http://www.sciencedirect.com/science/article/pii/B9780750668439000147.
Gordon B, Callan P, Vickers C. WHO guidelines for drinking-water quality. [Internet]. Vol. 38, WHO chronicle. 2008. 564 p. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid= 4053895&tool=pmcentrez&rendertype=abstract%5Cn
http://www.who.int/water_sanitation_health/dwq/fulltext.pdf%5Cnhttp://whqlibdoc.who.int/publications/2011/9789241548151_eng.pdf?ua=1
Navarro MO, Gaitan S, Duque ME. Determinación de Scherichia coli Y coliformes totales en agua por el método de filtraciòn por membrana en Agar Chromocult. Vol. 3, Ideam. 2007.
OMS. Guidelines for Drinking-water Quality [Internet]. Vol. 23, Atención Primaria. 2006. Available from: http://201.147.150.252:8080/xmlui/bitstream/handle/123456789/1262/Investigao_e_evoluo.pdf?sequence=1
Hernández García J. Potabilidad del agua en Acueductos de San Juan y Martínez Provincia Pinar del Río. 2009- 2010. Rev Ciencias Médicas Pinar del Río [Internet]. 2012;16(3):39–50. Available from: http://scielo.sld.cu/scielo.php?cript=sci_arttext&pid=S1561-31942012000300006&lang=pt
Prest EI, Hammes F, van Loosdrecht MCM,Vrouwenvelder JS. Biological stability of drinking water: Controlling factors, methods, and challenges.Front Microbiol. 2016;7(FEB):1–24.
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